A scientist runs an SDS-PAGE experiment two separate times on a protein with a molecular weight of 200 kD. For the first experiment he treats the protein with SDS only and for the second experiment he treats the protein with both SDS and a reducing agent. If the first gel yields one band showing a molecular weight of 200 kD and the second run yields one band showing a molecular weight of 25 kD, the researcher should conclude that

Answer:

This protein consists of 8 subunits of equal size linked by disulfide bonds

Explanation:

The sodium dodecyl sulfate-polyacrylamide gel (SDS-PAGE) is a technique widely used in molecular biology laboratories to separate proteins with molecular weights between 5 and 250 kDa. SDS is an anionic detergent used to denature proteins before electrophoresis. SDS can denature proteins by altering non-covalent bonds such as hydrogen, hydrophobic and ionic interactions, but they cannot cleave disulfide bonds. In this case, reducing agents (e.g.,  β-mercaptoethanol or dithiothreitol) have been used to cleave disulfide bonds.

In which direction is heat transferred?

PLEASE HELP!!!!
Explain what causes a muscle to go into rigor mortis. Your answer should include the circumstances which cause it, as well as why those circumstances cause the effect (what is happening in the muscle at a molecular level which causes the stiffness). ​

Emilia Burton is an 18-year-old high school student who began to experience weight loss despite a ravenous appetite and resulting increased dietary intake. She has to make frequent trips to the bathroom to urinate and has difficulty concentrating on her work because of fatigue. She drinks large volumes of coffee to help with a constant dry mouth and to combat her fatigue. At a clinic appointment, it was noted that her weight has dropped from 140 lb to 128 Ib.
a) What is the probable diagnosis of this patient? What information from the above scenario would support your diagnosis?
b) What laboratory test(s) should be performed? Which result(s) would support your diagnosis? What immediate and long-term therapy will Emilia need to manage her disorder?
c) Emilia should be aware of the potential acute and long- term complications of her condition. What are they?

Introspection is one of the many sleeping disorders experienced
by people with sleep apnea.
Select one:
True
False​

19. Fresh poultry should be rejected when:

A. It has been shipped in plastic wrap
B. It is slightly sticky to the touch
C. There is no discoloration
D. The receiving temperature is 38°F

Please Help!!!!
Muscles intended for large, powerful contractions contain llx muscle fibers. true or false​

Which of the following events occurs in DNA replication in prokaryotes?
a. Replication starts from a single point and proceeds in two directions until the entire chromosome is copied.
b. Replication starts from multiple points and proceeds in two directions until the entire chromosome is copied.
c. Regulatory proteins bind to all of the nucleotides on the DNA molecule.
d. Enzymes “unzip” the DNA molecule by breaking ionic bonds between base pairs.

The diagram below shows the crossing of a tall plant in a short plant tall is dominant which of the genotypes of the parents plants

A cluster of cases of legionellosis have occurred in your neighborhood. People with the illness have cough, fever, shortness of breath, chest pain and sometimes diarrhea. The illness is caused by the bacterium Legionella pneumophila, a facultative intracellular parasite that survives in certain ameba. The organism has an absolute requirement for L-cysteine. Typically, the organism is grown on buffered charcoal yeast extract (BCYE) agar. In some labs, samples containing suspected L. pneumophila are cultured on traditional BCYE medium as well as BCYE medium lacking L-cysteine. How would L. pneumohila be identified in this situation

Which of the following is not a function of the bacterial cell wall?

A. Provide the cell with shape and rigidity
B. Prevent the cell from lysing
C. Protect the cell
D. Provide the cell with locomotion

You perform an experiment in which chromatin is isolated from sea urchin sperm cells and briefly digested with micrococcal nuclease. When the chromatin proteins are removed and the resulting purified DNA is analyzed by gel electrophoresis, you observe a series of DNA fragments that are multiples of 260 base pairs in length (that is, 260 bp, 520 bp, 780 bp, and so forth). a) Although these results differ somewhat from the typical results discussed in the chapter, explain why they still point to the likely existence of nucleosomes in this cell type. b) What can you conclude about the amount of DNA that is associated with each nucleosome

Select all that apply.
Which of the following are true about a block of ice at -10°C as you continue to apply heat.


Its temperature will rise continuously until it completely melts.
Its temperature wil rise until reaching o°C.

Its temperature will not rise above -10°C until it melts. Its

temperature will remain at 0 G until all the ice melts.​

in what parts of the cycle do you think phosphorus spends the most time​

Determine the rate of oxygen consumption at each temperature for comparison. Then divide the higher rate by the lower rate to obtain the difference (ratio) between the two temperatures. Round your answer to the nearest 0.1. It can be concluded that mouse respiratory rate (increases or decreases) when temperature is lowered. In this experiment there was a fold difference in respiratory rate at the two temperatures.

Write one scientific question about the organism in
the photo. Sunflowers

A scientist is observing a new species of organism. She observes that from generation to generation many of the organisms’ offspring have adaptations that their parents did not have. This organism is most likely reproducing through_______.

a. sexual reproduction
b. budding
c. asexual reproduction
d. binary fission

pls help will give brainliest

The mutations responsible for the dark fur color in the Arizona mice were absent from the three different populations of New Mexico mice. No Mc1r mutationswere associated with dark fur color in the New Mexico populations. These findings suggest that adaptive dark coloration has occurred at least twice in the rock pocket mouse and that these similar phenotypic changeshave different genetic bases. How does this study support the concept that natural selection is not random

3. Microphages are a type of white blood cells and part of the immune system's response to defend the body against viruses. What do microphages do?​

What practice helps scientists avoid bias in their findings

The normal real behavior of a person or an animal can be
understood by naturalistic observation.
Select one:
True
False​

The diagram below represents a sample of a sedimentary rock viewed under a
microscope. Which part was formed first?

which are characteristics of epithelial cells? Select two options.( Answer Needed ASAP)​

How Green Plants Make Food
(a) ______ from the sun, (b) ______, the green coloring in the leaf of the plants combine with (c) _________ from the soil and (d) _________from the air to produce (e) ________ in the leaf. The air that enters the leaf contains carbon dioxide and oxygen. But the leaf needs only the carbon dioxide. Thus, (f) _________is given off as waste product.​

How do bacteria multiply again? Use the key terms genetic information, DNA, split in two, and exponential growth.

4. Calculate the kinetic energy of a Hollywood star running from the paparazzi. The star's mass is 45.0 kg, and the star is running at
a velocity of 3.00 m/s.
a) 203 J
b) 303.75 J
c) 405 J
d) 67.5 J

This equipment can be used for plowing ,planting,cultivating,mowing soil and pulling farm machinery,what is it?​

What factor causes global wind patterns

These are carbohydrates -rich vegetables EXCEPT
a. Tubers
b. nuts
c. seeds
d.roots​

Cellular Respiration and Energy