Rapid lateral flow assays based on the quantification of magnetic nanoparticle labels for multiplexed immunodetection of small molecules: application to the determination of drugs of abuse

Answers

Answer 1
A rapid lateral flow immunoassay

A rapid lateral flow immunoassay is presented that uses carboxyl-modified superparamagnetic nanoparticles as labels that can be quantified by highly sensitive multi-channel electronic readers. The approach is generic in that it is likely to be applicable to numerous small molecules. The method permits both single- and multiplex assays at a point-of-need without sample pretreatment. It is user-friendly and offers attractive characteristics demonstrated here for detection of morphine, fentanyl and methamphetamine in . The competitive immunoassay uses commercially available reagents that do not require special permissions. After migration of sample, the lateral flow test strips are subjected to an alternating magnetic field at two frequencies. The response from the nanolabels is readout at a combinatorial frequency from the entire volume of a porous immunochromatographic membrane by the magnetic particle quantification technique.

application to the determination of drugs of abuse.

Estimating the detection time of a drug in urine is complex because of many different influencing factors and the lack of experimental data. Detection times vary depending on dose and route of administration, metabolism and characteristics of the screening and confirmation assays. Using a cut-off value of 1000 ng/mL, urinary samples can be positive for amphetamine for up to 5 days after intake of the drug.

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Related Questions

A femur is a leg bone found in a human skeleton. When evaluating the human body's levels of organization, a femur is best classified as —


A.
an organ, because it is part of the skeletal system.
B.
an organ system, because it is composed of many cells.
C.
an organelle, because it is composed of many tissues.
D.
an organism, because it is part of the skeletal system.

Answers

Answer:

Option A

Explanation:

The femur is classified as an organ, because it is part of the skeletal system

The level of organization is the order that includes cells, tissues, organs, organ systems, and organisms. The femur is a leg bone which is an organ which is a part of organ system called the skeletal system.

What is skeletal system?

The basic framework of body is skeletal system, which is consists of bones, connective tissue, tendons, ligaments, etc.

The four main parts of this system include:

Bone.Ligament.Tendon.Joint.

The femur is a leg bone which is an organ that is a part of an organ system called the skeletal system.

Thus, the correct option is A.

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PLEASE ANSWER ALL QUESTIONS! THANKS!

Which of the following statements about salinity is true?
Question 1 options:

Ocean water near areas with low evaporation has higher salinity.


Ocean water in regions with high levels of precipitation has higher salinity.


Ocean water near rivers has a lower salinity.


Ocean water in areas with high humidity has a higher salinity



How are latitude and temperature related?








Question 2 options:

Lower latitudes will have warmer water because it is closer to the equator.


Higher latitudes will have warmer water because it is closer to the equator.


Higher latitudes will have warmer water because it is closer to the poles.


Lower latitudes will have warmer water because it is closer to the poles


How does salinity vary with freezing and melting?









Question 3 options:

Both freezing and melting decrease salinity.


Both freezing and melting increase salinity.


Freezing decreases salinity, while melting increases salinity.


Freezing increases salinity, while melting decreases salinity.


How does salinity vary with evaporation?









Question 4 options:

When water evaporates, it takes salt with it, increasing its salinity.


When water evaporates, it leaves salt behind, increasing its salinity.


When water evaporates, it leaves salt behind, decreasing its salinity.


When water evaporates, it takes salt with it, decreasing its salinity.

Answers

Answer:

that guys answers are all wrong except for #3

Explanation:

i took the quiz and got 1/4

If a chromosome comes apart because it is not well attached at the center, 1 point
then there is most likely a problem with the

centromere
chromatids
chromatin
gene

Answers

Answer: The most problem is likely with chromatin

Explanation:

If a chromosome comes apart because it is not well attached at the center, The most problem is likely with chromatin.

What is chromosome ?

A chromosome is  a thread-like structure present in the nucleus of cells such as both prokaryotic and eukaryotic cell and it is made of a molecule of DNA (Deoxyribonucleic acid) and histone proteins.

It is composed of three components, DNA, proteins and RNA,  amount of the DNA content ranges from 30 to 40 %, the amount of proteins from 50 to 65%, and small amount of RNA from 1 to 10%.

Two types of protein present in DNA such as histone and non-histones proteins, histone constitute large amount (90% of total protein), whereas non-histone is present in small amount (around 10% of total protein).

The histone protein is basic, whereas, the DNA is acidic, so it can be neutralized; DNA in chromosome  is present in two forms such as Euchromatin and Heterochromatin.

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1. Which statement is NOT true?

a. Metals tend to lose electrons and form cations, or positive ions.

b. Nonmetals tend to gain electrons and form anions, or negative ions.

c. Ionic bond is formed between two metals or two nonmetals.

d. Ionic bond is formed between a metal and a nonmetal.

Answers

Answer:

c.Ionic bond is formed between two metals or two non metals.

The answer is C-Lonic bond is formed between two metal or two nonmetals

Which type of cell is considered the least complex?

Answers

Answer:

tissues.

organs.

organ systems.

organism.

Explanation:

Answer:

the answer would be tissues

What is the definition for polyploidy?

Answers

containing more than two homologous sets of chromosomes.

Make a concept map with the following terms:
Stimulus, function of relation, response senses or receptors, touch, smell, environment, individual, stimulus, taste, hearing, sight, processing center, nervous system, somatic, central nervous system, peripheral nervous system, neurons, synapses, sensory, brain, motor, interneurons, electrical, chemical, spinal cord, autonomic, voluntary and involuntary actions

Please is for today :(

Answers

Answer: igu bro, ok first u add my bbrother to class and then u subrtarct the ugly kids and you omneof them so dieeeee

Explanation:

Arrange the following concepts from simplest to most complex: Chromosomes, DNA, Genome, Gene

Answers

Answer:

DNA -> Gene -> Chromosome -> Genome

Explanation:

A genome is an organism's complete set of DNA including all of its genes. Each genome contains all of the information needed to build and maintain that organism. Chromosomes are the threadlike structures located inside the nucleus of animal and plant cells. A gene is the basic physical and functional unit of heredity.

The material and components that are vital for the growth and functioning of an organism that gets inherited from parent to offspring are the components of genetic information.

From simplest to complex they are arranged as:

DNA ⇒ Gene ⇒ Chromosome ⇒ Genome

DNA is the deoxyribose nucleic acid and is the genetic material that transports the instruction for development and function for growth.

Gene is a unit of hereditary that codes for gene products.

DNA molecules packed as a thread-like structure is called chromosomes.

The entire set of genes that pass from parent to offspring is called the genome.

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what reaction is degradative

Answers

Simple sugar dehydration–fragmentation products, such as derivatives of furans, pyrones, cyclopentenes, carbonyl compounds, or acids

---

please brainliest :)

Answer:

Degradation reactions reduce the number of atoms in a molecule through a specific type of chemical transformation.MARK ME AS BRAINLIST

What is the process by which an organism undergoes a dramatic change in form called?
1. Morphology
2. Metastasis
3. Photosynthesis
4. Metamorphosis

Answers

Metamorphosis through which the insect develops by four distinct stages: egg, larva, pupa, adult. A type of metamorphosis in which an organism's transformation is so dramatic that it is difficult to recognize the relationship between the larva and adult form.

Option 4) Metamorphosis

Answer:

4. Metamorphosis

Disadvantages of smoking

Answers

Answer:

lung cancer

Explanation:

people die everyday due to smoking so we must take action also smoking has no benifits only losses

Due to the size of the Universe and the number of galaxies and stars in it, scientists must devise indirect ways to study it. Which of these represents the best way for scientists to study the Universe?

Answers

Answer:

A.  Scientists should create a graph showing a comparison of the relative sizes of all the galaxies and stars.

Explanation:

Due to the size of the universe and the number of galaxies and stars in it, scientists must use different methods for the purpose of the study, such as the analysis of cosmic microwave background radiation, gravitational lensing, etc.

What is the significance of the cosmology studies?

Scientists can conduct cosmology research in a variety of ways, including using computer simulations to model and predict the evolution of the universe and all of its materials, using gravitational lensing to measure the distribution of dark matter, and so on. Apart from that, the universe can be studied by using telescopes at various wavelengths,

Hence, due to the size of the universe and the number of galaxies and stars in it, scientists must use different methods for the purpose of the study, such as the analysis of cosmic microwave background radiation, gravitational lensing, etc.

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Estimate the number of cells that belong to each phase of the cell cycle. Then, explain how you made your decision and describe what occurs at each phase of mitosis.

Answers

Answer:

well, it starts from one parent cell, every complete phase makes. at least 4 and then i. think those can multiply

Explanation:

science animal cell, please help thanKS ASAP

Answers

Answer:

a (so 6)

Explanation:

I hope this is helpful :)

What is the the process responsible for the synthesis of proteins and mention the cellular structure where the genetic program responsible for this synthesis is located.

Answers

Answer: Translation

Explanation:

During the transcription the double stranded DNA act as a separate template  strand to produce mRNA molecule. The mRNA molecule encode for amino acid chain that collectively forms the protein. The ribosomes are the sites of protein synthesis the mRNA get attached to the ribosome. Within the ribosome the rRNA molecule instruct the catalytic steps for the synthesis of protein. The amino acid sequence or chain gets stitched together to form the protein.

3. What might happen to the ecosystem if this population were removed?

Answers

Answer:

More predators kill more prey, which, along with food scarcity, decreases the population.

Explanation:

Answer: ''Population Explosions The most obvious result of the removal of the top predators in an ecosystem is a population explosion in the prey species. ''

Explanation:

What will happen if you cross a white (bb) rabbit with another white (bb) rabbit?

Answers

Yes that’s absolutely right

The codon GAG becomes GTG due to a point mutation in the DNA, affecting the structure of the protein hemoglobin. What effect does this mutation have on the individual? WILL GIVE BRAINLIEST
The individual suffers from sickle cell anemia.

The individual suffers from myotonic dystrophy.

The individual suffers from Fragile X syndrome.

The individual suffers no negative effects.

Answers

i believe it to be the first one

Answer:

The individual suffers from sickle cell anemia.

Explanation:

Deformed red blood cells occur in 1 in 500 African Americans. This is caused due to a gene mutation called point mutation that alters the structure of the red blood cell. This alteration affects the cell's ability to carry oxygen.

Soil erosion can be BEST prevented by

- Heavily watering the vegetation on the slope

- Increasing the slope of the land by adding more soil

- Building terraces into the sides of a slope

- removing grass from the steepest slope.

I need help!!

Answers

Answer: Following are some of the methods of soil erosion prevention: Plant trees on barren lands to limit erosion of soil. Add mulch and rocks to prevent the plants and grass underneath to prevent soil erosion. Mulch matting can be used to reduce erosion on the slopes.

Answer:

Building terraces into the sides of a slope

Im stuck in this question if you know please comment

Answers

Answer:

C

Explanation:

meiosis increases the genetic variability due to mixing of genetic material between chromosomes.

it produces genetic variation by a process known as recombination.

please answer please, If we have a male hemophiliac (such as Leopold) marry a normal female, is there any way to have sons who have hemophilia? Provide a justification for your response.

Answers

Yes, hemophilia is a genetic condition, which means it can be genetically passed down to a male child, even if only one parent is a hemophiliac. The condition is also more common in male children, and is a sex-linked recessive disorder, and is carried on the X chromosome. The mother could also have the gene without actually having the disorder, so she could possibly be a carrier without knowing it, so it is possible for their son to have hemophilia, even if the dad is the only one with the gene.
Hope this helps!!

What sort of evidence would support at least one of the components of cell theory ? a Rocks and other non-living things are not composed of cells b A microscope is necessary to make observations of nearly all cells c Cells are observed converting one form of energy into another d The origin of all cells can be traced to previous or pre existing cells

Answers

Answer:

D

Explanation:

The correct answer would be that the origin of all cells can be traced to previous or pre-existing cells.

According to the cell theory which is made up of three parts;

The cell represents the basic unit of all life.All living organisms are made up of cells.Cells do not arise spontaneously, but only from pre-existing cells.

Hence, the discovery that the origin of all cells is traceable to previously existing cells supports the latter part of the cell theory.

The correct option is, therefore, D.

How can you determine the number of bonds an atom can make

Answers

Answer:

The number of bonds for a neutral atom is equal to the number of electrons in the full valence shell (2 or 8 electrons) minus the number of valence electrons. This method works because each covalent bond that an atom forms adds another electron to an atoms valence shell without changing its charge.

The North Pole and the South Pole are

A:Classified as tundra biomes

B: Not home to any animals

C: not classified into major biomes.

D: Part of Aquatic Ecosystems​

Answers

D part of aquatic ecosystems

Answer:

A

Explanation:

classified as tundra biomes

all of the following are nucleus acids , except
a - adenine
b - iodine
c - guanine
d - cytosine

Answers

Answer:

iodine

Explanation:

Iodine is a chemical not a base. There are only 4 nucleus acids and these are adenine, thymine, cytosine and guanine.

Put "Gene Pool" in a sentence

Answers

Answer:

A gene pool is a collection of all the genes in a population. This can be any population - frogs in a pond, trees in a forest, or people in a town.

Explanation:

True or False
Prokaryotic cells generally have a higher surface area to volume ratio than eukaryotic cells.

Answers

Answer:

true I think I just guessed

TRUE : Prokaryotic cells  generally have a higher surface area to volume ratio than eukaryotic cells

Eukaryotic cells are larger and complex when compared to prokaryotic cells which makes its surface area to volume ratio to be smaller than that of prokaryotic cells. cells are classified based on the arrangement  of their cell structures.

Prokaryotic cells are found in smaller organisms like Bacteria while Eukaryotic cells are found in larger organisms like Humans and Plants.

Hence we can conclude that based on the complexity and size of the Eukaryotic cell, the prokaryotic cells generally have a higher surface area to volume ratio.

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PLZ HELP I"LL GIVE BRAINLIEST

Answers

Answer:

gotchu

Explanation:

1. His symptoms consist of difficulty walking and an abnormal gait (pattern of movement such as walking, running, etc)

2. a. one purpose of the blood test was to test his creatine kinase enzyme to see if there were any medical conditions connected to the way he was walking and why it was abnormal

b. the other purpose is to be sure that he has something wrong with his gait. If he does have a medical condition, it was best to see if he had it early on to treat it faster

3. the function of dystrophin gene connects to the cytoskeleton of a fiber which has to do with brain function; we need that to walk. For DMD, that is a condition that alters the way people walk.

4. DMD is inherited from family's genes, so he got it from his birth family probably from his dad's part of the family as DMD effects men more than women

5. It is pretty likely as this medical condition is inheritably passed on. It is likely that his grandchild will get DMD

6. To treat DMD to the best of the ability since there isnt a cure, they could participate in physical therapy and steroids

Describe how you will estimate the rate of photosynthesis.

Answers

Explanation:

there are various methods to measure photosynthesis: Uptake of CO2 by plants: Since CO2 is needed for photosynthesis, measuring how much of it is taken up by the plants gives us information on how much of photosynthesis is happening. Release of O2: The amount of O2 produced during photosynthesis can be measured.

Estimation or measuring the rate of photosynthesis can be done by:

by the production of oxygenthe uptake of carbon dioxideIndirectly by an increase in biomass.

The rate of photosynthesis is a gross measure of the rate at which a plant captures radiant energy and fixes it in organic carbon compounds. It can be estimated by:

Depletion of the substrate that is carbon dioxide, has been used or how much water is used.the accumulation of oxygen is a measure of the rate of reaction.measure the rate of photosynthesis in leaves by observing the release of oxygen

Thus,

Estimation or measuring the rate of photosynthesis can be done by:

by the production of oxygenthe uptake of carbon dioxideIndirectly by an increase in biomass.

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The population of most cities are numbered in _____________

Answers

Explanation:

muchos miles, y aveces cientos de miles

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Before the switch is closed in the figure, the potential across the capacitor is 200 V. At some instant after the switch is closed, the instantaneous current is 0.70 A. What is the energy in the capacitor at this instant 1.) Read Hamlet and take notes on the play.2.) Determine what stance (your thesis) you are taking.3.) Do additional research about the stance you want to take on the play to get ideas of how to further your stance and support it (research is required - at least two reliable sources). 4.) Write your thesis statement.5.)Write your alphanumeric outline beneath your thesis. It must have at least three main points with two sub-points each.Please note that when parenthetically citing a play, you put the act, scene, and line number into the citation. Example: (Hamlet 2, 3, 17-20). This example would indicate the play Hamlet, act 2, scene 3, lines 17-20. Each of the following decreases total stockholders' equity except a cash dividend. liquidating dividend. stock dividend. all of these decrease total stockholders' equity. An employee worked for 8 hours on 2 days, 6 hours on 1 day, and 4 hours on 2 dayWhat is the average number of hours the employee worked per day?A.) 6.0B.) 6.5C.) 7.0D.) 7.5E.) 8.0 Which sentence best describes the tone of the passage from the book compared to the passage from the radio brodcast How can e-waste lead to health and safety issues? The u.s. centers for disease control and prevention (cdc) estimates that approximately 87.5 percent of u.s. adults are:_____. Which marketing organization or entity is not part of traditional marketing channels or supply chains? Mike jacobs is planning to lease an automobile. in an effort to minimize the price of the vehicle being leased, mike should compare the ____________ at different leasing companies. The Fannie Mae 1004 form is intended to be used to report an appraisal on modular or panelized homes. Which term describes how fast work is done?A. EfficiencyB. Mechanical advantageC. ForceD. Power According to the general equation for conditional probability, if P(AB) = and P(B) = 2, what is P(A/B)? 12 O A. 3/ . 4 O c. 2/ C. 5 O D. 9 Question 9 of 10What is the solution to this equation?x+12= -5O A. x = 17O B. x= -7O C. x = 7O D. x= -17 use the sum to product formula to find exact values: if cos255 - cos195 = squrtroot a/ 2 then a= Duress occurs when a. a party gives notice of refusal to be bound by an agreement. b. an improper threat is made to force another party to enter into a contract. c. both parties contract based on the same factual error. d. the enforcement of a contract results in unconscionability. A nurse is assessing a client who has gestational diabetes. Which of the following manifestations are findings of hyperglycemia Which of the following choices represents a balanced chemical equation? At the end of Sunrise Over Fallujah, Robin tells Marla he loves her, but she doesn't say it back right away. Which quote identifies how she feels about Robin's declaration of love?- "I'm thinking real hard on you."- "...when people shoot at you...you automatically love everybody that's ducking down with you."- "I'm sorry," she said, "I'm so sorry."- "There was nothing that needed to be said." As soon as possible, you need to learn from Lori in Shipping and Receiving whether she and her colleagues can fulfill a large, time-sensitive customer order. If she cannot, you need her advice on where you can get it done. Tin and oxygen can combine to form two compounds of different combining proportions. Resulting formulas of these compounds includea. Sn2O and SnO2 d. SnO and SnO3b. SnO and SnO2 e. SnO2 and SnO4c. SnO4 and SnO???